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ORIGINAL ARTICLE
Year : 2020  |  Volume : 8  |  Issue : 1  |  Page : 1-5

Phenotypic and genotypic detection of malaria parasite among patients attending Aminu Kano Teaching Hospital, Kano, Northwest Nigeria


1 Department of Medical Microbiology and Parasitology, Faculty of Clinical Sciences, College of Health Sciences, Bayero University, Kano, Kano State, Nigeria
2 Center For Dry Land Agriculture, Bayero University, Kano, Kano State, Nigeria

Correspondence Address:
Mr. Abdulrazak Muhammad Idris
Department of Medical Microbiology and Parasitology, Faculty of Clinical Sciences, College of Health Sciences, Bayero University, Kano, Kano State
Nigeria
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/njecp.njecp_28_19

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Introduction: Malaria parasite (Plasmodium species) is one of the most important parasitic infections affecting people of all age groups throughout tropical countries wherever suitable hosts are found. The parasites are transmitted to man by the malaria vector, the female Anopheles mosquito during blood meals. Among all the members of the genus Plasmodium, Plasmodium falciparum is the most prevalent and common malaria species worldwide, especially in Africa and other developing countries. Aims and Objective: The present study aimed at the phenotypic and genotypic detection of malaria parasite among patients attending the General Outpatient Department of Aminu Kano Teaching Hospital, Kano, Northwest Nigeria. Material and Method: A total of 456 samples were collected from patients that voluntarily consent to participate in the study. Blood samples were randomly collected by trained personnel by vein puncture; and thick and thin smear was made on a clean glass slide and left to air dry. The smear was stained with Giemsa stain and observed under × 100 microscopic objective lens. A total of 41 (9.0%) positive malaria parasite smears were obtained with 0–9 years. Result: From 41 positive malaria parasite smears obtained by microscopic examination, 15 were randomly selected and confirmed by polymerase chain reaction (PCR) and ten were selected from negative microscopic smears. Eight (53.3%) out of the 15 positive smears and 3 (30.0%) from the negative smears were confirmed to be PCR positive. Conclusion: The finding of the study confirmed the presence of malaria parasite among the patient group in the study area using both phenotypic and genotypic detection techniques.


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