Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login 
ORIGINAL ARTICLE
Year : 2013  |  Volume : 1  |  Issue : 1  |  Page : 23-27

Methanolic crude leaf extract of Ocimum gratissimum reverses phenylhydrazine-induced anemia in albino wistar rats


1 Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, College of Medicine, University of Nigeria, Enugu Campus, Enugu, Enugu State, Nigeria
2 Department of Pharmacology and Therapeutics, Faculty of Medical Sciences, College of Medicine, University of Nigeria, Enugu Campus, Enugu, Enugu State, Nigeria
3 Department of Physiology, Faculty of Medical Sciences, College of Medicine, University of Nigeria, Enugu Campus, Enugu, Enugu State, Nigeria
4 Department of Medical Rehabilitation, Faculty of Health Sciences and Technology, College of Medicine, University of Nigeria, Enugu Campus, Enugu, Enugu State, Nigeria
5 Department of Medicine and surgery, Faculty of Medical Sciences, College of Medicine, University of Nigeria, Enugu Campus, Enugu, Enugu State, Nigeria

Correspondence Address:
Nubila Thomas
Department of Medical Laboratory Sciences, University of Nigeria, Enugu Campus, Enugu State
Nigeria
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2348-0149.123959

Rights and Permissions

Background: The therapeutic and culinary applications of Ocimum gratissimum leaf have been practiced in Central and West Africa for decades. This study was designed and conducted to investigate the haematinic and haemopoietic activities of phenylhydrazine-induced anaemia in Albino wistar rats. Materials and Methods: Twenty-four (24) Albino wistar rats were grouped into four (A, B, C and D) with six rats per cage. Groups B, C and D served as the test groups, while group A was the control. The test groups were gavaged with graded doses (4, 8 and 12mg/kg body weight respectively) of Ocimum gratissimum methanolic crude leaf extract once daily for 9 days. Two (2) mls of venous blood was collected from the ocular plexus from each animal into tripotassium ethylene diamine tetra-acetic acid anticoagulant bottle. This was used to determine complete blood count using automated haematology autoanalyzer (Sysmex KX-21N). Results: Red blood cell (RBC) mean value recorded a dose and duration-related statistically significant decrease (P < 0.05) in group D on day 3. However, there was statistically significant increase (P < 0.05) in the RBC indices. In addition, total white blood cell (WBC) recorded a marginal increase in the mean values throughout the study period though not statistically significant (P > 0.05). Furthermore, lymphocyte recorded both dose and duration-related statistically significant increase (P < 0.01). Conclusion: From the result of this study, it can be concluded that the methanolic crude leaf extract of Ocimum gratissimum possesses both haemopoetic and haematinic potentials which are dose and duration-related.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed2672    
    Printed119    
    Emailed0    
    PDF Downloaded261    
    Comments [Add]    

Recommend this journal